Advances in Biochemical Engineering, Volume 9 by V. L. Yarovenko (auth.)

By V. L. Yarovenko (auth.)

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13. It is, therefore, coneluded that the growth rate of C. interrnedia on hydrocarbon is a function of the hydrocarbon pool. The hydrocarbon pool is related to the specific concentration of hydrocarbon per unit cell mass in the medium by the term S • X -1. Thus the relationship between the specific growth rate and the specific concentration of hydrocarbon per unit cell mass, S • X - l , was investigated. The results are shown in Fig. 24, which indicates that the saturation kinetics is valid for the specific growth rate and the specific concentration of hydrocarbon per unit cell mass.

31 ] and Nakahara et al. [33]). C. intermedia IFO 0761 adhers tightly to the surface of the hydrocarbon drop and the microbes on the hydrocarbon drops form clumps during growth, as shown in Section 2. The transfer of substrates other than hydrocarbon was investigated in the growth which accompanied clump formation. This was done so as to assess which substrate-transfer step limits the growth rate, knowledge of which is important for the formulation of a kinetic model of growth. The uptake rate of 3~-HaPO4 from a medium containing n-tetradeeane and glucose was compared with that of a glucose medium without n-tetradecane.

4. Hydrocarbon Pool . . . . . . . . . . . . . . . . . . . . . 5. Growth Kinetics of Microorganisms with Low Affinity for Liquid Hydrocarbon . . . 6. Growth Kinetics of Microorganisms with High Affinity for Liquid Hydrocarbon . . . 1 Transfer of Substrate During Clump Formation Accompanied Growth . . . . . 2 The Effect of Operating Conditions on the Growth Rate . . . . . . . . . 3 Kinetic Model for Growth . . . . . . .

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